A Polygalacturonase from Citrus Leaf Explants
نویسندگان
چکیده
منابع مشابه
Activity of pectin esterase and cellulase in the abscission zone of citrus leaf explants.
The activity of pectin esterase and cellulase in abscission of citrus explants was studied. No relation was established between pectin esterase and abscission, while cellulase activity was markedly increased before abscission and for a certain period after excision. IAA and cycloheximide delay abscission and cellulase activity, while ethylene and, to a lesser extent, GA(3) accelerate them. Appl...
متن کاملAbscission of citrus leaf explants: no correlation with naphthaleneacetic Acid conjugation in the abscission zone.
The role of alpha-naphthaleneacetic acid (NAA) in the control of abscission in Citrus (Citrus sinensis L. Osbeck) leaf explants and its conjugation were studied in non-aged and 24-hour-aged explants. Dipping non-aged explants in 1.5 micromolar NAA for 15 minutes immediately after excision did not delay abscission whereas 150 micromolar NAA effectively delayed it. As incubation time was prolonge...
متن کاملThe Effects of pH, Ionic Strength, and Ethylene on the Extraction of Cellulase from Abscission Zones of Citrus Leaf Explants.
The solubility of cellulase extracted from the abscission zones of citrus leaf explants (Citrus sinensis L. Osbeck) in sodium phosphate buffer depends on the pH of the extracting solution and, to a lesser extent, on the ionic strength. By increasing molarity from 0.01 to 0.16, the solubility of cellulase increased from 51% to 89% at pH 6.1 and from 70% to 98% at pH 7. In all cases, residual cel...
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Background and Aims: CBLVd has been reported from northern of Iran previously. The aim of this study was evaluating new viroid variant from asymptomatic citrus trees of Fars province and comparing with its other isolates. Materials and Methods: In this study a number of citrus trees without symptoms were sampled and subjected to RNA extraction followed by RT-PCR, cloning and sequencing of ...
متن کاملCharacterization of a Polygalacturonase from Trichoderma harzianum Grown on Citrus Peel with Application for Apple Juice
A polygalacturonase (PGase) was purified from Trichoderma harzianum , grown on citrus peel, by anion exchange and size exclusion chromatographies. The enzyme had molecular mass of 29 kDa. The extent of hydrolysis by PGase was decreased with increasing of pectin esterification degree. m m ax K and V , using polygalacturonic acid as substrate, were 1.42 mg and 0.66 ìmol reducing sugar, respective...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1974
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.53.2.312